Objective: This study aims to evaluate the effectiveness of a virtual reality (VR) module in enhancing knowledge and response skills for hospital kitchen fire emergencies. Materials and Methods: A VR module was developed and integrated into a hospital’s kitchen fire response training procedure in April 2022. A total of 87 participants, including senior and novice dietitians, interns, food court managers, chefs, and kitchen staff, used the VR module. All participants underwent pretest and posttest assessments to determine their fire response knowledge and satisfaction levels. Subsequently, participants with (n = 15) and without (n = 25) VR training participated in an offline 3-day fire drill in 2023, and their pretraining and post training test scores were compared. Results: All participants exhibited significant improvements in their posttest scores (p < .05), with kitchen staff demonstrating the greatest improvements (38.8 points, p < 0.001). The average satisfaction score among all participants was 97.6 (extremely satisfied). A significant increase was observed between the pretest and posttest scores of participants with and without VR training who participated in the 3-day fire drill (7.8 ± 3.6, p = 0.037). Conclusion: The proposed VR module is an effective time- and cost-saving tool that can aid in hospital kitchen fire emergencies. It significantly enhances knowledge and response skills while increasing teaching quality and participant satisfaction.