In order to maintain cardiorespiratory fitness and health, the training of aerobic capacity is important to athletes and general population. High-intensity interval training is a strategy of exercise, and more studies were about the cardiorespiratory fitness and performance of athletes. The purpose of this article was to discuss the effect of high-intensity interval training on cardiorespiratory fitness and health. High-intensity interval training could improve maximum oxygen uptake (VO2max) significantly. Some studies showed that the improvement of VO2max in high-intensity interval training is even better than in moderate-intensity continuous exercise with longer time. The ability of cardiac output and the number of mitochondria increased significantly after training. Total body fat and central abdominal fat decreased significantly. The control of metabolic syndrome and bood glucose improved significantly after training.