| 中文摘要 |
本報告採用精確、簡易、經濟、省時的色帶固著電氣泳動法，對各種屠畜新鮮肉品鑑別真偽以杜絕摻偽事件之發生，進而保護消費者大眾，達到維護國民健康之目的。各種鑑別方法，年來海內外壘有報告出現，唯費時，不經濟者較多（如免疫沈降法），亦或觀察不易（如電氣泳動法）。本報告特綜合上述各家之長處，改進其缺失，將各泳動讚加以染色，使其易于觀察，達到快速鑑別之初衷，其中又以纖維凝膠，瓊脂凝膠，分別經PonceauS及Amido Black 10 B之染色較適于一般實驗室之用，無毒、安全，泳動譜較易于觀察且可做為永久保存之用。
A major advance in detecting the fresh meat adulteration has been the development of use staining fixation electrophoresis, a simple, precise, rapid and economic mehtod, for the qualitative identification of animal proteins as to species. The skeletal muscle extract, was applied on thin layer gel electrophoresis, 225 voltage 30min cellulosic strip and 45 mA 150 min agar noble gel, respectively. Then staining fixation by dye amido black 10 B or ponceau S. The method requires a maximum times of only 3~6 hr, in addition, the electrophoregraphy was easy to observed. |
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