紅斑性狼瘡血清對常人自然殺手細胞成熟過程的影響

The Influence of Lupus Sera on the Maturation of Normal Natural Killer Cells

將從十四位健康男性血液中所分離之Fc接受器陽性及陰性之淋巴細胞，分別先經紅斑狼瘡血清或常人血清處理然後再培養7天。在第一、四、七天分別以單株抗體Anti-Leu-7(HNK-1)與0KT3標示。以計算被試驗之淋巴細胞中淋巴細胞亞群之分佈情形。目的在觀察(1)自然殺手細胞是否由Fc接受器陰性之淋巴細 胞所產生。(2)在7天之培養過程中自然殺手細胞是否有轉變為T細胞之現象，(3)紅斑性狼瘡血清對自然殺手細胞之影響。結果顯示從Fc陰性之淋巴細胞中，沒有產生自然殺手細胞之現象，同時亦無發現自然殺手細胞轉變為T淋巴細胞之情形。自然殺手細胞中有Fc陽性與陰性之次群體，兩次群體中之不成熟自然殺手細胞之百分比有明顯之差異。淋巴細胞在培養前先經過紅斑狼瘡血清之處理，結果對自然殺手細胞及不成熟殺手細胞之百分比分佈無影響。顯示紅斑狼瘡血清對自然殺手細胞之成熟過程無影響。在七天培養過程中，具有Leu-7 (HNK-1)標誌之細胞無明顯之變化，所以若想多了解自然殺手細胞成熟之過程及其與T細胞之關係，則需同時以Anti-Leu-7, Anti-Leu 11兩種單株抗體來作研究。

Lymphocytes with or without Fc receptors (FcR⁺ or FcR⁻) from 14 healthy males were preincubated with or without lupus sera and then cultured for 7 days. Lymphocyte subpopulations were enumerated with monoclonal anti-Leu-7 (HNK-l) and OKT3 on day 1, day 4 and day 7. The study was designed to see: (1) Whether natural killer (NK) cells are generated from FcR⁻ lymphocytes? (2) Do NK cells shift to T cells during the 7- day- culture? and (3) The influence of lupus sera on the maturation of NK cells. Our results showed that no evidence indicated the generation of NK cell from FcR⁻ lymphocytes. There was also no evidence of shifting NK cells to T cells. Different subsets of NK cells, FcR⁺ & FcR⁻, with different ratio of functional immature NK cells were found. Preincubation with lupus sera did not cause the change of percentages of NK cells and functional immature NK cells during the culture period. It indicates lupus sera do not affect the course of NK maturation. NK cells with Leu- 7 (HNK-1) surface marker failed to show any significant change in a 7- day- cuI(ure. Concomittant study of lymphocyte subpopulations with anti- Leu- 7 (HNK-l), anti- Leu- 11 and OKT3 is needed to provide more informations in NK cell maturation and the relationship between NK cells and T cells.