英文摘要 |
The interaction between normal human granulocytes and microcrystal- line monosodium urate monohydrate (MSUC) was studied by an assay method of lucigenin-dependent chemiluminescence. Various proteins were adsorbed onto the crystals by incubation in order to clarify the requirement of opsonization for MSUC to initiate an inflammatory reaction. Among them, normal human serum (NHS) displayed the highest opsonic activity with tbe chemiluminescence of 15.6 ± 2.0 mV which was, however, markedly diminished to 6.4 ± 0.6 mV if it was decomplemented. Nevertheless, partial reconstitution of the decomplemented NHS with a samll amount of guinea pig complement, whose opsonic activity was quite low (33 ± 0.9 mV), could restore the reaction to 16.4 ± 1.9 mV. This synergetic effect on the opsonic activity was also seen in the interaction between PMNs and IgG-adsorbed crystals which was originally only moderately high (4.7 ± 0.4 mV), but showed 3 times augmentation of chemiluminescence after the guinea pig complement proteins were added. IgA and IgM were weak in opsonic activity and not influenced by complement. Human albumin was unable to adsorded onto the crystal surface. It was therefore possible that cooperation between IgG and complement proteins were essential for PMNs to Initiate an acute episode of gouty arthritis. |