Endothelium-Dependent Higenamine-Induced Aortic Relaxation in Isolated Rat Aorta

The pharmacological action of higenamine in isolated rat aorta was investigated. Although the beta-adrenoceptor antagonist propranolol (1 × 10-5 M) completely blocked the beta-adrenoceptor agonist higenamine in inducing a positive chronotropic activity in isolated mouse atria, the higenamine-induced aortic relaxation was not completely antagonized by this concentration of propranolol. The present data also demonstrate that the higenamine-mduced aortic relaxation was attenauted in the absence of endothelium. These findings suggest that the beta-adrenoceptor specificity to higenamine in aorta is different from that of beta-1 in atria; moreover, the beta-adrenoceptors sensitive to higenamine are mainly located in the endothelial layer.