DETERMINATION OF PARISHIN,PARISHINS B AND C IN TRADITIONAL CHINESE MEDICINAL PREPARATIONS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

High performance liquid chromatographic methods were established for the determination of parishin. parishins B and C in three kinds of the traditional Chinese medicinal prescriptions. including Gastrodiae Rhizoma: Shuen-Feng-Iun-Chi-San, Ban-Shia-Bai-Ju-Tian-Ma-Tang and Tsan-Siang-Tian-Ma-Tang. The samples were analyzed with an Inertsil ODS-2 reversed phase column by gradient elution with varied 0.1% (v/v) phosphoric acid-acetonitrile as mobile phases and detected at UV 222 nm. Ethoxybenzamide was used as an internal standard. Regression equations were derived showing linear relationships (correlation coefficients: 0.9976-0.9999) between the peak area ratios of each marker (parishin. parishins B and C) to ethoxybenzamide and concentration. The recoveries of parishin, parishins B and C from the Chinese medicinal preparations were 102.0-104.1 %, 102.9-105.0% and 103.2-104.0%. respectively.