異常血清檢體之免疫球蛋白E定量測定

Quantitative Determination of Immunogobulin E in Hemolyzed and Lipemic Specimens

溶血與脂血往往會干擾實驗室檢驗的數值。為了瞭解溶血與脂血對於total IgE的影響，以Phadia immunoCAP100、Immunilite1000以及Beckman-Coulter ACCESS Immunoassay System來進行測定與比較。結果顯示三種系統，其溶血或脂血都與正常血清具有高度的相關（相關係數>0.9），其中以Phadia immunoCAP100的相關性最高（溶血組：0.999，脂血組：0.998）。而在不同濃度的比較上，DPC immunilite1000的相關性隨濃度而降低，而Phadia immunoCAP100與Beckman-Coulter ACCESS Immunoassay System的相關性則無明顯的變化。雖然系統不同，三種不同系統在測定脂血與溶血的toal IgE上都有良好的表現。

Hemolyzed and lipemic specimens sometimes interfere with test results, especially when detecting the total IgE of a specimen. Phadia immuno CAP100, Immunlite 1000 and Beckman-Coulter ACCESS Immunoassay System were used to try and understand how hemolyzed and lipemic specimens interfered with the measurement of total IgE. The experimental results from the three different machine assays showed that hemolyzed/ lipemic specimens and normal serum specimens show a high degree of correlation with each other (interference coefficient >0.9). When the three systems were compared, the Phadia immunoCAP 100 method was the most stable and most suitable system for detecting total IgE in the two types of specimens (coefficient for the hemolyzed samples was 0.999 and for the; lipemic samples was 0.998). However, the IgE DPC immunilite 1000 method showed a greater efficiency when detecting small amount of IgE. Furthermore, in conclusion, all three systems showed good performance when detecting IgE in detecting lipemic and hemolytic specimens.