In response to global climate change and Taiwan’s Pathway to Net Zero Emissions in 2050, Kaohsiung Municipal Siaogang Hospital implemented the “Precision Carbon Reduction through Green Surgery” In this hospital, operating rooms are considered the most energy- and resource intensive units. This initiative aimed to systematically promote carbon reduction and workflow optimization. Through a cross-departmental governance framework, the hospital implemented targeted strategies in four key domains: improving energy efficiency, reducing anesthetic gas emissions, promoting resource circularity, and digitizing administrative processes. When this initiative was implemented, the total carbon emissions from operating rooms decreased from 211,459.34 to 147,686.35 metric tons of CO2 equivalent, representing an overall reduction of 30.16%. In addition, the electricity and water consumption of high-pressure boilers decreased by more than 50%, and operations were made fully paperless in both operating rooms and the Department of Anesthesiology. Moreover, implementing sustainable governance measures was not found to compromise healthcare quality. On the contrary, by enhancing process standardization, improving information transparency, and mitigating risks, the initiative contributed to improved patient safety and care efficiency. This case demonstrates that the green operating room model is feasible and replicable, and this study may serve as a practical reference for healthcare institutions seeking to advance their net-zero transformation policies while maintaining healthcare quality.