This study aims to study the relationship between teacher calling, learning burnout and career hope among students from the art university of northern Taiwan of Grades. The research method was questionnaire survey and 278 participants participated in this research. The research tools included: "Basic Personal Information ", "Teacher Calling Scale", "Learning Burnout Scale" and "Career Hope Scale". The obtained data were analyzed by descriptive statistics, One-way ANOVA, Pearson Correlation, and Multiple regression analysis. The research results are as follows: There were no significant differences in teacher calling, learning burnout, and career hope among art university pre-service teachers of grades.Except for "Egoism" of teacher calling and learning burnout, all the variables have reached significant correlation levels; teacher calling had significant positive effect on career hope; Except for "Emotional and physical exhaustion", learning burnout had significant negative effect on career hope.