月旦知識庫
  1. 熱門:
 
首頁 臺灣期刊   法律   公行政治   醫學   財經   社會學   教育   其他 大陸期刊   核心   非核心 DOI文章
查看詳細全文
篇名
評估BluePointTM MycoID及MALDI-TOF MS在分枝桿菌生長指示管系統進行非結核分枝桿菌鑑定
並列篇名
Evaluation of BluePointTM MycoID and MALDI-TOF MS for Identification of Nontuberculous Mycobacteria from the Flagged Mycobacterium Growth Indicator Tube system
作者 黃心妮李建岳陳湘鈞鍾孟妤劉佳龍黃昱璁李岱芬
中文摘要
本研究評估BluePointTM MycoID及MALDI-TOF MS(Bruker Biotyper)在陽性Mycobacterium Growth Indicator Tube(MGIT)(BACTEC, MGIT 960 system, Becton-Dickinson, Sparks)系統中菌種鑑定情況,並以16S–23S rDNA ITS定序確認不相符結果,藉以探討最合適的快速非結核分枝桿菌鑑定方法。本次收集的91支陽性MGIT中,BluePointTM MycoID和M ALDI-TOF MS(Bruker Biotyper)準確度分別為97.8%及91.2%。其中11%的MGIT檢體BluePointTM MycoID僅能鑑定到Mycobacterium spp,可知BluePointTM MycoID適合快速提供臨床菌種名稱,但有11%的檢體因菌量不足或菌種資料庫有限,僅能鑑定為Mycobacterium spp。MALDI-TOF MS雖能鑑定較多分枝桿菌菌種,但需等待菌落生成及專業人員經驗累積,因此時效性較差。
英文摘要
BluePointTM MycoID and MALDI-TOF MS(MALDI Biotyper)were evaluated to find accurate and rapid identification of nontuberculous mycobacteria from the positively flagged Mycobacterium Growth Indicator Tube(MGIT)system(BACTEC, MGIT 960 system, Becton-Dickinson, Sparks). The discordant results were checked by 16S–23S rDNA ITS sequencing. Among 91 consecutively positive MGIT cultures, the accuracy of BluePointTM MycoID and MALDI-TOF MS(Bruker Biotyper)were 97.8% and 91.2% respectively. BluePointTM MycoID identified 11% of 91 consecutively positive MGIT cultures only as Mycobacterium species. Although it could provide faster identification of single or multiple NTM species/genus directly from the positively flagged MGIT system, BluePointTM MycoID identified 11% of all samples as Mycobacterium species due to the limited NTM targets included. MALDI-TOF MS(MALDI Biotyper)could identify more species t han those from BluePointTM MycoID, it is time-consuming and need professional experience.
起訖頁 92-99
關鍵詞 Nontuberculous MycobacteriaBluePointTM My coIDMALDI-TOF MS
刊名 生物醫學暨檢驗科學雜誌  
期數 202206 (34:2期)
出版單位 台灣醫事檢驗學會
該期刊-上一篇 Plasma Lipid Profiling Increased Cardiometabolic Risks in Acute Myeloid Leukaemia Patients Pre- and Pro-chemotherapy
該期刊-下一篇 乙型鏈球菌(Group B Streptococcus)引起健康成人化膿性關節炎(septic arthritis)
 

新書閱讀



最新講座


優惠活動




讀者服務專線:+886-2-23756688 傳真:+886-2-23318496
地址:臺北市館前路28 號 7 樓 客服信箱
Copyright © 元照出版 All rights reserved. 版權所有,禁止轉貼節錄