使用鉺雅鉻雷射促進鞣花酸之經皮吸收

Enhancement of transdermal delivery of ellagic acid by Er：YAG laser pretreatment

鞣花酸（Ellagic acid）是一種天然來源的酚類化合物，廣泛存在於石榴、莓果和山核桃等食材中。由於具有抗氧化及抑制黑色素的能力，近年被廣泛應用於抗老化和皮膚美白領域。鉺雅鉻雷射（Er：YAG laser）因其可設定能量精確磨除角質層，且施打後恢復期較短，近年常被應用於美容醫學中以增強外用藥物的穿透效果。本研究以鉺雅鉻雷射當作促進試驗藥物的工具，來證實是否能有效提升鞣花酸的藥物穿透能力，同時嘗試找出可實際應用於臨床試驗的最佳雷射能量。本研究使用的試驗皮膚為豬皮，試驗皮膚會先由不同能量的鉺雅鉻雷射前處理後，再利用垂直式經皮吸收擴散試驗裝置（Franz cell）接續分析之穿透濃度，之後計算其穿透速率（flux）及促進倍率（enhancement ratio，ER），來比較不同雷射能量對促進鞣花酸經皮吸收能力之成效。結果顯示，經由鉺雅鉻雷射處理的組別，使用能量範圍為2.5 J／cm2~7.5 J／cm2與未處理的組別比較下，其鞣花酸促進倍率為17~63倍。本研究證實，經由鉺雅鉻雷射前處理後，可顯著提升鞣花酸之經皮吸收能力，而最適雷射之能量設定為5 J／cm2。

Ellagic acid is a naturally occurring polyphenolic compound abundant in foods including pomegranates，berries，and pecans. Owing to its antioxidant properties and its ability to inhibit melanin synthesis，it has been widely used in anti-aging and skin-whitening treatments in recent years. The erbium-doped yttrium aluminum garnet（Er：YAG）laser，recognized for its capacity to precisely ablate the stratum corneum with adjustable energy settings and relatively short recovery times，has become increasingly popular in aesthetic medicine to enhance the transdermal delivery of topical agents. This study investigates the use of the Er：YAG laser in facilitating drug penetration and whether it effectively enhances the transdermal absorption of ellagic acid. Additionally，it aims to identify the optimal laser energy setting suitable for potential clinical application. Porcine skin served as the experimental model. The skin samples were pretreated with various energy levels of the Er：YAG laser and subsequently analyzed using a vertical Franz diffusion cell system to measure the percutaneous absorption of ellagic acid. The flux and enhancement ratio（ER）were calculated to evaluate the efficacy of different laser energy levels in promoting transdermal absorption. The results demonstrated that laser-treated groups（fluences ranging from 2.5 J／cm²to 7.5 J／cm²）showed a 17- to 63-fold increase in enhancement ratio compared with the untreated control group. This study confirms that pretreatment with an Er：YAG laser significantly enhances the transdermal delivery of ellagic acid，identifying 5 J／cm²as the optimal energy setting for achieving the best enhancement effect.