使用鉺雅鉻飛梭雷射促進維生素C衍生物之經皮吸收

Skin pretreatment with a fractional Er:YAG laser promotes the transdermal delivery of vitamin C derivative

維生素C-2-葡萄糖苷(ascorbic acid 2-glucoside, AA2G)是一種維生素C衍生物，結構經由修飾過後性質安全穩定，有抑制黑色素的作用，因而可做為美白成分。本計畫以鉺雅鉻飛梭雷射(fractional Er:YAG laser)當作促進試驗藥物的工具，來證實是否能有效提升維生素C-2-葡萄糖苷的經皮吸收能力，同時找出可利用於臨床試驗的最佳雷射能量。本研究使用體外動物實驗模式，試驗的皮膚則為豬皮。試驗皮膚會先由不同能量的鉺雅鉻飛梭雷射前處理後，再利用垂直式經皮吸收擴散試驗裝置(Franz cell)接續分析之穿透濃度，之後計算其穿透速率(flux)及促進倍率(enhancement ratio, ER)，來比較不同雷射能量對促進維生素C-2-葡萄糖苷苷經皮吸收能力之影響。結果顯示，經由鉺雅鉻飛梭雷射處理的組別，使用能量依序為2.5 J/cm²、3.8 J/cm²、5 J/cm²和6.3J/cm²與未處理的組別比較下，其維生素C-2-葡萄糖苷促進倍率為15~18倍、21~25倍、36~39倍和40~43倍。本研究證實，鉺雅鉻飛梭雷射可顯著提升維生素C-2-葡萄糖苷之經皮吸收能力，而最適雷射之能量設定為5 J/cm²。

Ascorbic acid 2-glucoside（AA2G）is a modified ascorbic acid derivative known for its safety and stability. It can inhibit melanin production, making it effective as a whitening ingredient. This study utilizes a fractional Er:YAG laser to enhance the transdermal absorption of AA2G, verify its a These authors contributed equally to this work. efficacy, and determine the optimal laser energy settings for clinical application. An in vitro animal model was employed, specifically using pig skin. The test skin was pre-treated with the fractional Er:YAG laser at various energy levels, followed by an analysis of penetration concentrations at six different time points using Franz cells. The flux and enhancement ratio（ER）were calculated to compare the impact of varying laser energy levels on the transdermal absorption of AA2G. The results indicate that compared to the untreated group, the ER for AA2G in the lasertreated groups with energies of 2.5 J/cm², 3.8 J/cm², 5 J/cm², and 6.3 J/cm²are 15-18 times, 21-25 times, 36-39 times, and 40-43 times higher, respectively. This study confirms that the fractional Er:YAG laser significantly enhances the transdermal absorption of AA2G, with the optimal laser energy setting being 5 J/cm².