月桃花與種子萃取物之抗氧化研究

Determination of Antioxidant Activity of the Flower and Seed Extracts of Alpinia zerumbet

月桃（Alpinia zerumbet）為臺灣常見的多年生草本植物，也是民間傳統廣泛利用的原生植物。月桃不僅擁有獨特的香氣，可用於製作料理，還擁有充滿韌性的葉鞘，可製作生活用品。本研究使用台灣原生之月桃花與種子部位為材料，利用乙醇、乙酸乙酯、正己烷萃取得到的粗萃物，利用清除DPPH自由基活性測試、總酚類化合物含量與總抗氧化能力試驗來探討萃取物之抗氧化性。研究結果顯示月桃花以屯鹿月桃（A. tonrokuensis）花苞抗氧化能力最佳，在清除DPPH自由基活性試驗中濃度於100μg/ml時DPPH自由基清除率達到74.11%，其總酚類化合物含量也是最高的，但總抗氧化能力試驗中屯鹿月桃花苞含量最高。月桃種子以使用乙酸乙酯萃取之花蓮地區月桃種子抗氧化能力最佳，在清除DPPH自由基活性試驗中濃度於100μg/ml時達到60.15 %。

Alpinia zerumbet is a common perennial herb in Taiwan and is also a native plant widely used in folk traditions. Not only does it have a unique fragrance that can be used for cooking, but it also has a tough leaf sheath that can be used as a household product. In this topic, uses the flower and seed parts of Alpinia zerumbet as materials, the crude extracts were extracted with ethanol, ethyl acetate and hexane, and the antioxidant properties of the extracts were investigated by using DPPH free radical-scavenging activity test, total phe-nolic content measurement and Trolox total antioxidant capacity test.
The results of the study showed that Alpinia zerumbet flowers have better antioxidant capacity than Alpinia zerumbet seeds. In the DPPH free radical-scavenging activity test, Alpinia tonrokuensis flower buds had the best ability to scavenge DPPH radicals, with a concentration of 74.11 % at 100μg/ml, and the highest total phenolic content, but was the second highest in the Trolox equivalent antioxidant capacity assay The seeds were the best in antioxidant capacity of ethyl acetate extracted seeds of Hualien region Alpinia zerumbet, reaching 60.15 % at a concentration of 100μg/ml in the DPPH radical scavenging activity assay.