利用同功酶和逢機放大多型性DNA評估台灣產山黑扁豆屬之一雜交種

Evaluation of a Natural Hybrid of Dumasia DC. (Fabaceae) from Taiwan Based on the Isozymes and RAPD Studies

本文利用同功酶和逢機放大多型性DNA研究苗栗野豇豆和台灣山黑扁豆之間的雜交種。雜交種不僅在形態上和同功酶三個基因座（Aat-1, Aat-2, Mdh-3）的等位基因條帶介於兩親本之間，且共有疑似親本二十四條逢機放大多型性DNA條帶。在同功酶上幾乎沒有新的等位基因產生，此雜交種可能是最近才產生而沒有足夠時間累積突變，但在DNA層次上發生少許突變。

This paper describes a natural hybrid, derived from the putative parent of D. miaoliensis and D. villosa subsp. bicolor. This hybrid is not only morphologically intermediate between its putative parents but also at three loci (Aat-1, Aat-2, Mdh-3) from isozymes data, and twenty-four RAPD bands represent a combination nature in its putative parental species. The nearly complete lack of novel alleles suggest that it is of recent origin and that insufficient time has elapsed for the accumulation of mutation at isozyme loci, but it has little mutations at the DNA level.