食用油脂及膠囊錠狀食品中迷迭香萃取物檢驗方法之開發   全文下載

Development of Test Method for Rosemary Extracts in Edible Oil and Food in Tablet or Capsule Form

迷迭香萃取物為我國准用之食品添加物，可使用於食品中作為抗氧化劑，使用限量以鼠尾草酸及鼠尾草酚總量計，限於食品製造或加工必須時使用。本研究係以液相層析串聯式質譜法（Liquid Chromatography-tandem Mass Spectrometry, LC-MS/MS）建立食品中鼠尾草酸與鼠尾草酚之檢驗方法。考量常添加迷迭香萃取物之產品，選取食用油脂、錠狀食品及軟膠囊食品為試驗基質，以乙腈振盪萃取，經離心後取乙腈層，再經過濾後以LC-MS/MS分析。本方法之確效試驗係於前述3種基質中，添加對羥苯甲酸丁酯之內部標準溶液2 mg/kg，再加入鼠尾草酚與鼠尾草酸各1及5 mg/kg，進行5重複試驗，鼠尾草酚平均回收率介於98.1 - 115.9%之間，變異係數介於1.2- 9.3%之間；鼠尾草酸平均回收率介於89.4 - 116.8%之間，變異係數介於2.5 - 12.0%之間，本方法之定量極限於鼠尾草酸及鼠尾草酚均為1 mg/kg。另以市售食用油脂、錠狀食品及軟膠囊食品等15件檢體驗證所建立之檢驗方法適用性，結果均未有基質干擾之情形，且檢出結果均符合相關規定。

The analytical method for determing rosemary extracts in foods by the liquid chromatographytandem mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) mode was developed. Dosage of rosemary extracts was estimated by the total amounts of carnosic acid and carnosol. The sample was extracted with acetonitrile, and the supernatant from high speed centrifugation was analyzed by LC-MS/MS. The recoveries of carnosic acid and carnosol spiked in oil, tablets and soft capsule samples at the concentrations of 1 and 5 mg/kg were investigated. The average recoveries of carnosic acid were between 98.1 to 115.9% with coefficient of variation between 1.2 to 9.3% and those of carnosol were between 89.4 to 116.8% with coefficient of variation between 2.5 to 12.0%. Both of the detection limits of carnosic acid and carnosol were 1 mg/kg. Fifteen commercial product samples were analyzed by this method. All the test results were in accordance with the regulation.