| 英文摘要 |
"In vitro culture of rice buds has been developed to induce tolerance to phosphinothricin（PPT）, i.e., the glufosinate herbicide. Treatment at stage I of tissue culture with PPT concentrations higher than 0.76μM inhibited the bud growth of rice cv. Koshihikari, with smaller buds being more sensitive. Through 28-day incubation, microshoot induction was most significantly stimulated by 5.0μM PPT, as compared with other concentrations; with higher levels resulting in plant death. At stage II of tissue culture, all rice plantlets were decapitated to 15 mm tall and incubated for 30 days in hormone-free medium containing 5.0μM PPT. Rice buds which were cultured PPT-free at the first stage, and followed by 5.0μM PPT treatment, generated a higher induction rate of microshoot than those treated at both stages. At stage II, plantlets that survived 30 days after treatment of 10.0μM PPT produced more microshoots than those treated with no or 5.0 mM PPT. While no significant difference in tolerance with a foliar-application of PPT at either 0.75 or 1.51 mM was observed in microshoot-derived plants which had survived from 9 different PPT treatment combinations, 11 plants could be referred as the PPT-tolerant and -susceptible plants, based on the both extremes of frequency distribution of injury index of all plants. Two tolerant plants and two susceptible plants have been selected by means of leaf segment assay." |
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