樟芝發酵菌絲體用於，預防乾眼症之功能性評估

Effect of Fermented Antrodia cinnamomea Mycelium Extract in the Prevention of Dry Eye Syndrome

樟芝（Antrodia cinnamomea）為臺灣特有的真菌之一，具有免疫調節及保護肝臟之生理活性。然而，目前尚未有樟芝應用於預防乾眼症的研究報導。本試驗利用液態發酵培養樟芝菌絲體，並評估其萃取物預防乾眼症之能力。試驗使用ICR雌性小鼠，隨機分為四組，分為對照組、誘導乾眼症組、低劑量組（10 mg/ kg bw）及高劑量組（100 mg/kg bw）。其中誘導乾眼症組分別以benzalkonium chloride（BAC）化學性及ultraviolet B（UVB）物理性誘導傷害。結果顯示，BAC化學性誘導傷害模式下，經由角膜外觀分析及中心角膜細胞組織切片可發現其可減緩角膜損傷，在淚液分析中淚液變化量增加，淚液品質提升。在UVB物理性誘導傷害模式下，由角膜外觀分析，可觀察到其可緩解角膜平滑度及損傷染色程度，在淚液分析中淚液變化量增加，淚液品質提升。綜上所述，樟芝發酵菌絲體萃取物具有預防化學性及物理性傷害之乾眼症的能力。

"Antrodia cinnamomea is one of Taiwan's unique fungi, and has been reported to exhibit physiological activities such as immune regulation and liver protection. However, the effect of A. cinnamomea on dry eye syndrome has not been studied. Hence, this study aims to use A. cinnamomea mycelia extract from submerged liquid fermentation and evaluates its effectiveness to prevent dry eye syndrome. ICR female mice used in this study were randomly divided into four groups including control, damaged, low-dose (10 mg/kg bw) and high-dose (100 mg/kg bw) groups. The damaged groups were induced by chemical BAC and physical UVB, respectively. Results showed that in the chemical BAC-induced model, A. cinnamomea administration is effective to reduce corneal damage via corneal appearance and tissue section analyses as well as to increase the quality and quantity of tear film components. In the physical UVBinduced model, A. cinnamomea exposure could alleviate corneal surface damage graded according to corneal smoothness and extent of lissamine green staining as well as to promote secretion of tear fluid. Taken together, the findings of this study suggested that A. cinnamomea mycelia extract has the potential to prevent dry eye syndrome caused by chemical and physical damages."