The 17th-century Zeelandia City located in today’s Anping area is an important research topic considering its role in the history of Taiwan and the Dutch East India Company. However, due to the limited information provided by historical sources, it remains difficult to carry out an extensive research on the spatial structure and elements of this city. Thanks to the discovery of the manuscript document of “1643 Estate Registers of Zeelandia” by the Dutch historian Menno Leenstra a few years ago, it now becomes possible to undertake an in-depth research on this city. This document brings to light the cadastral information in the mid-1640s, consisting of 395 building lots in total. More than 75% of these plots are recorded with the information of their dimensions of length and width, the names of their owners, and the streets where they are located. The paper presents the research process of the reconstruction of the ground plan of Zeelandia City in the mid-1640s according to this document and also the discussion of the distribution of land ownership in this city based on the reconstructed ground plan. First of all, “1643 Estate Registers of Zeelandia” is examined so as to acquire the useful information about the streets, blocks and building plots. Second, two historical drawings-the “1635 Town Plan of Zeelandia City” and the “1648 Bird’s Eye View of Zeelandia” are analyzed in order to get the approximate measurements of the streets and blocks in the mid-1640s and clarify their spatial relationship. Third, the 1927 cadastral map of Anping is scrutinized to find out whether the urban texture of the 17th century is still observable in the present day. Fourth, two physical traces of Zeelandia City are confirmed in today’s Anping area, and they are used as the crucial reference for locating the 17th-century streets and blocks. Lastly, according to theabove-mentioned information, the ground plan of Zeelandia City in the mid-1640s can be properly constructed. During the process of the reconstruction, some important historical documents of the Dutch East India Company are also taken into consideration. In the reconstructed ground plan, the forms, locations and measurements of the streets, blocks, building plots and coastline of Zeelandia City in the mid-1640s as well as the plan of Fort Zeelandia are all clearly presented. Moreover, the distribution of land ownership is mapped and visualized on this ground plan for further analysis and discussion. The outcome of this research can also be used as the basis for more research topics related to the spatial development and transition of Zeelandia City or Anping area and for the comparison of the ideas of town planning in Dutch overseas colonies. Last but not the least, this research can provide helpful information for the future archeological survey in Anping area.