If nurses can carry out fistula function assessment correctly and distinguish the characteristics of fistula failure clinically, the quality for patients and nurse-patient relationships could be enhanced. This project is intended to improve the correct assessment of fistula functions by the hemodialysis nursing staff. An analysis of status quo revealed that the low accuracy of fistula functional assessment by the hemodialysis nursing staff may be due to a lack of knowledge, skill proficiency and appropriate assessment tools. Therefore, a communication platform and a standard on fistula functional assessment were developed, along with an assessment tool and evaluation guidelines; the nursing staff also participated in occupational training on fistula evaluation. After this project has been implemented, accuracy of fistula functional assessment improved from 62.5% to 86.6%; the fistula assessment awareness score increased from 63.5 to 93.6; the early treatment of fistula abnormality improved from 31% to 83.3%. The results showed that occupational training, clinical operating guidelines and routine monitoring can effectively improve the accuracy of the nursing staff thus gaining acceptance from dialysis patients on the quality of fistula care.