Acceptable quality of water filtered with reverse osmosis (RO) is a critical component for ensuring the health of hemodialysis patients. A routine water quality monitoring exercise conducted on 22 July 2016 found abnormal amounts of bacterial colonies and prompted the improvement program. Analysis of this assessment found that the primary issues were: ambiguous standards for RO conduit disinfection, lack of instruments to detect a high concentration of bleach, defective design of the water outlet for the water source, absence of auditing mechanisms and awareness among personnel. Remedial measures taken included: group meetings, acquiring RO system management experience of other hospitals through benchmarking, establishing conduit disinfection protocol, holding educational training, purchasing disinfectant concentration monitors, replacing water outlet to streamline the disinfection treatment process and generating a conduit disinfection verification checklist. The aim is to produce improved RO water quality that would reduce the abnormal rate of bacterial colonies (>50 cfu/ml) from 20% to 10%, to increase the precision rate of RO conduit disinfection process from 66.7% to 100%, and to increase the precision rate of water sampling process in RO bacterial monitoring from 83% to 100%. This project has effectively resolved RO water quality issues and achieved the improvement of the safety for hemodialysis patients. Relevant units may use this study for future reference.