食品中游離胺基酸、葡萄糖胺及牛磺酸同步分析方法之建立暨市售產品小型抽測結果

Simultaneously Determination of Glucosamine, Taurine, and 20 Amino Acids in Foods and the Surveillance Report

胺基酸是構成蛋白質的基本單位，依結合基團差異約有20種常見胺基酸。凱氏氮粗蛋白檢測儀可檢測總含氮量並推估總蛋白質含量，惟現行產品種類及型態眾多，胺基酸種類及含量差異大，致含氮係數判斷困難，又機能性食品常添加葡萄糖胺及牛磺酸，易造成分析干擾，各界使用分析方法立論基礎時有差異，爰擬建立食品中胺基酸、葡萄糖胺及牛磺酸之同步分析方法，以利各界參考使用。本研究顯示檢液經柱前衍生化後，以Agilent Poroshell HPH-C18（3.0×100 mm，2.7μm）管柱搭配光二極體陣列檢出器進行分析，可於15分鐘內分離20種胺基酸及葡萄糖胺與牛磺酸等化合物。以所建立之檢驗方法抽測市售產品共計16件，產品基質囊括液狀（含乳水）、粉狀（含乳粉）、膠囊狀（含油狀基質軟膠囊）及錠狀（含發泡錠），檢驗結果顯示有2件產品未達標示值之80％，檢測項目分別為精胺酸及葡萄糖胺，其餘產品檢測值均達標示值80％以上，本次市售產品抽測標示符合程度達87.5％，顯示此批食品之製造業者於此類產品中游離胺基酸、葡萄糖胺及牛磺酸之自願性標示成分品管措施良好。 Amino acids are the building blocks of proteins. There are twenty amino acids occur commonly in nature. In some functional foods or dietary supplements, glucosamine and taurine are usually accompanied with amino acids. Therefore an analytical method for simultaneous detection of 20 amino acids, glucosamine, and taurine in foods is needed. Sample extract was analyzed by high performance liquid chromatography after extraction and derivation. The analytical time was in 15 minutes achieved by using Waters ACQUITY UPLC system with an Agilent Poroshell HPH-C18 column (3.0 × 100 mm, 2.7 μm) and a photodiode array detector. A surveillance of 16 commercial samples with different matrices such as milk, milk powder, oil capsules, and effervescent tablets was conducted. The results showed the detected values in 14 samples were higher than 80% of the labeled values in their packages, which were in compliance with the regulation. However, 2 samples were found containing lower arginine and glucosamine, respectively, to their 80% of labeled values failed to meet the regulation. The pass rate was 87.5% that seemed the self-management of most manufacturing companies performed well.