食品中微量維生素E檢驗方法探討

STUDY ON THE ANALYTICAL METHOD OF VITAMINE E IN FOODS

探討維生素E檢驗之前處理，比較其在皂化中添加水份，對嬰兒配方奶粉之分析結果會造成何種影響，結果發現，如添加水份，則使 dl-α-Tocopherol Acetate 不能完全轉換為 dl-α-Tocopherol。利用高效液相層析儀連接紫外光檢測器波長280 mm和螢光檢測器 EM 330nm，Ex 290nm 同時檢測比較，發現螢光檢測器對 α-Tocopherol 之感度較紫外光檢測器高出約25倍。依探討後之維生素E前處理方法及採用高達液相層析儀具螢光檢測器EM330，Ex290 nm，對三類不同食品進行回收試驗，結果平均回收率於嬰兒配方奶粉為100.4%，於特殊健康食品為98.5%，於食用油脂食品為97.0%，儀器最低檢出限量為0.2 ug/ml。

The conditions of pretreatment for vitamine E analysis, especially the effect of the saponification of infant formulas with or without water added on the analytical result was studied. It was found that dl-x-Tocopherol acetate would not be completely transformed to dl-α-tocopherol if water was added to sample before saponification. High performance liquid chromatoqrophy with a U.V. detector was compared with that with a fluore scente detector for vitamine E detection. The results showed that fluorescence detector (EM 330 nm, EX 290 nm) had higher sensifivity forα-Tocopherol, while UV detector had higher sensitiving for its acetate form. Recovery analysis for three groups of foods were performed. Average recoveries forα-Tocopherol added to infant formalas, health foods and edible fats and oils were 100.4%, 98.5% and 97.0%, respectively. The lowest detection limit forα-Tocopherol by the above mentioned instrument was 0.2 ug/ ml.