以回應曲面法探討溫度、pH質和氯化鈉含量對 Aeromonas hydrophila DNA group 1 生長之影響

Response Surface Model for Predicting the Effects of Temperature, pH and Sodium Chloride Content on the Growth of Aeromonas hydrophila DNA Group I

本研究利用回應曲面法探討 Aeromonas hydrophila DNA group 1 之生長影響因子，以其 ATP 含量表示該菌之活菌量，影響因子則包括起始 pH 值（4.6, 5.3, 6.3, 7.3, 8.0），培養溫度（22, 25, 30, 35, 38℃）和氯化鈉含量（0.8, 1.5, 2.5, 3.5, 4.2%） 。結果發現該菌其培養之起始 pH 值對 ATP 含量影響並不大，而培養溫度和氯化鈉含量之影響則較為顯著。由結果得知該菌在下述條件時其活菌量最多（即 ATP 含量最高）：當培養24小時，培養溫度20.3℃～24.5℃，起始 pH 值6.2～6.3，氯化鈉含量0.80～0.88%；培養48小時，培養溫度25.8～27.4℃，起始 pH 值5.8～6.2，氯化鈉含量0.87～0.88%。

The combined effects of temperature (22, 25, 30, 35 and 38℃), Sodium Chloride Content (0.8, 1.5, 2.5, 3.5 and 4.2%), and initinal pH (4.6, 5.3, 6.3, 7.3 and 8.0) on the growth of A. hydrophila DNA group 1 were studied in tryptic soy broth using response surface model. We use ATP measurement as a biomass indicator. The results showed that the bacterial A TP content was not significantly affected by the initial pH value, while the temperature and sodium chloride content were markedly affected The optimal growth conditions were found to be temperature 20.3-24.5℃, initial pH 6.2-6.3, sodium chloride content 0.80-0.88% after incubated 24 hours; temperature 25.8-27.4℃, initial pH 5.8-6.2, sodium chloride content 0.87-0.88% after 48 hours of cultivation.