台灣地區養殖魚類抗生類殘留之調查

AN INVESTIGATION OF ANTIBIOTIC RESIDUES OF CULTURED FISHES IN TAIWAN

民國75年11月至76年5月自台北市、高雄市及台灣省北、中、南、東等四區之傳統市場、超級市場抽購養雞魚類270件（包括鰻魚98件、鯉魚科43 件、鱸鱒魚42件、虱目魚45件、吳郭魚42件）檢驗氯黴素、羥四環素、氯四環素等三種抗生素，結果氯黴素、羥四環素均未檢出，氯四環素檢出4件（鰻魚2件、鱸魚1件、虱目魚1件），檢出率1.5%。四環素類之試驗微生物為 Bacillus cereus var. mycoades ATCC 11778 以圓筒平板法及生物自析法定性定量，檢出限量羥四環素為O.05ppm，氯四環素為O.Olppm，平均回收率分別為90.2%及84.5%；氯黴素之試驗微生物為 Escherichia coli NIHJ，以圓筒平板法及液相層析法定性定量，檢出限量1.25ppm，液相層析最低感度可達 1ng，平均回收率89.3%。

From Nov. 1986 to May 1987. 270 cultured fishes (including 98 eels, 43 cyprinids, 42 perciformes and Rainbow Trout, 45 milk fishes, 42 sarotherdons) were purchased from the fresh markets and supermarkets in Taiwan area and analyzed for oxytetracycline (OTC), chlorotetracycline (CTC), chloramphenicol (CP). The results showed that OTC and CP were undetected in all samples and CTC was detected in 4 sample which included: 2 eels, 1 perciform, 1 milk fish. The cup-and-plate method and TLC-bioautography with Bacillus subtilis var. mycoides ATCC 11778 as the test organism were employed for the TCs assay and the average recoveries were 90.2% (OTC) and 84.5% (CTC). As little as 0.05 pm (OTC) or 0.01 ppm (CTC) was detectable. The cup-and-plate method with E. coli NIHJ as the test organism and HPLC were employed for the CP assay and the average recovery was 89.3%. As little as 1.25 ppm was detectable by bioassay. The detecting limit by HPLC was 1 ng.