乳品、禽肉、豬肝中林可黴素(Lincomycin)及健牠黴素(Gentamicin)殘留之檢驗方法探討

METHOD FOR DETECTION LINCOMYCIN AND GENTAMICIN RESIDUES IN MILK, CHICKEN AND SUINE-LIVER

本報告係以0.1MpH8.0磷酸緩衝液及系統性的溶媒抽取法作選擇性抽取，將各抽取液點在TLC板上，經展開後，再以已接菌種之方型培養基板與之接觸，培養後視所形成抑制圈之Rf值作為鑑別殘留抗生素種類之依據；同時將檢液以圓筒平板法作生物檢定，依據抑制圈之大小來定量。將林可黴素及健牠黴素添加於食品後分別以溶媒或緩衝液抽取，以圓筒平板法檢測其限量分別如下：林可黴素：0.1～0.5μg/g（依不同或抽取液而不同，健牠黴素O.1μg/g，林可黴素以溶媒和緩衝溶液抽取之平均回收率分別為54.5～77.5%及28.5～81.7%。健牠黴素以溶媒和緩衝溶液抽取之平均回收率則分別在56～83.7%及46.5～52.5%，兩種抗生素之回收率皆以溶媒抽取者較高。生物自析法是以纖維薄層鋁片經7%氯化銨和0.5MpH7.0磷酸緩衝液展開較佳，各種抗生素可依不同之Rf值區分開來。林可黴素及健牠黴素之最低感度分別為0.3及0.05μg。

A microbiological procedure was evaluated to detect gentamicin and lincomycin residues in food. Antibiotic residues were extracted from samples with methanol and methanol-HCL ( 98+2 ) . The methanol extract was further extracted with chloroform to isolate a group of antibiotics. The extracts were spotted onto TLC plates and developed by suitable solvent systems. Developed plates were then placed on set medium seeded with Bacillus subtilis. A bioautograph was formed after incubated at 37℃ for 17 hours. Recoveries of lincomycin added to milk, chicken and swine liver ranged at 4.5-77.5% and 28.5-83.7% with solventsystems and buffer extraction respectively. while recoveries of gentamicin were 56-84.7% with solvent system and 46.5% - 52.5% with buffer extraction. The lowest detectable doseof lincomycin and gentamicin with solvent system and phosphate buffer extraction were 0.1-0.5 and 0.1 µg / g respectivly. The TLC-bioautographic method with cellulose aluminum sheet by 7% NH4C1 or pH7, 0.5 M phosphate buffer were found to be the best developing system for these two antibiotics. The lowest detectable dose of this method was 0.3 µg lincomycin and 0.05 µg gentamicin.