防己藥材中馬兜鈴酸分析方法之建立與含量調查

DETERMINATION OF ARISTOLOCHIC ACID IN FANGCHI RADIX

以高效液相層析儀，分析防己中馬兜鈴酸I、II （aristolochic acid I、II）之含量。採用C18逆相層析管柱，移動相為乙腈 -1.25% 醋酸溶液（40：60），檢測波長為254 nm，得到良好之分析結果。馬兜鈴酸I、II 在濃度0.63~40.00µg/mL、0.40~25.76 µg/mL 範圍內，利用上述分析技術進行對照標準品馬兜鈴酸I、II 之分析得線性迴歸方程式及相關係數（r）分別為 Y=1.06E-05X-0.28（r=0.9998）, Y = 8.28E - 06X + 0.12 （r=0.9998），顯示良好線性關係。馬兜鈴酸I之同日內及異日間之相對標準偏差為1.17%及1.71%，添加回收率為103.08±3.42%，馬兜鈴酸II之同日內及異日間之相對標準偏差為1.28%及1.70%，添加回收率為103.13±3.68%。價購市售防己藥材二十件，利用上述高效液相層析法分析，馬兜鈴酸I含量介於0.030~0.192 %之間，平均含量為0.086%，馬兜鈴酸II含量介於0.017~0.118%間，平均含量為0.043%。

A high performance liquid chromatographic method for determination of aristolochic acid il and II in Fangchi Radix was established. The samples were analyzed by HPLC on a Cosmosil 5C18-AR column and UV detector (254 nm) with acetonitrile-1.25% acetic acid (v/v) (40:60) as a mobile phase at a flow rate of 1.0 mL/min. The regression equations of aristolochic acid I and II were Y = 1.06E-05X-0.28 (r=0.9998) and Y = 8.28E-06X+0.12 (r=0.9998), respectively. The intraday and interday relative standard deviations of aristolochic acid I were 1.17% and 1.71%, and the recovery was 103.08 ± 3.42%. The intraday and interday relative standard deviations of aristolochic acid II were 1.28% and 1.70%, and the recovery was 103.13 ± 3.68%. 20 samples of Fangchi Radix were analyzed. Results showed that average contents of aristolochic acid I and II in samples were 0.086% and 0.043%, and the ranges