雞肉、雞蛋及雞肝中乃卡巴精之殘留分析

Determination of Nicarbazin Residues in Chicken Muscle, Egg and Liver

利用高效液相層析法分析雞肉、雞蛋及雞肝中乃卡巴精殘留量之檢驗方法業已建立。乃卡巴精以乙腈自檢體中萃取，經過濾，以正己烷去雜質後濃縮，再以氧化鋁淨化，最後利用高效液相層析儀分析定量。檢體中添加乃卡巴精0.1、0.2、0.4及0.8ppm時，其回收率分別為雞肉89.8～93.8%，雞蛋89.9～93.3%及雞肝90.4～92.5%，變異係數皆小於10%。本方法在上述三種檢體中的最低檢出量低達0.02ppm。本研究於民國八十年一月至八十年五月間於全省檢市售雞肉檢體30件，雞蛋檢體47件及雞肝檢體25件，經檢測結果，發現乃卡巴精殘留雞肉之檢出率為33.3%，殘留量0.02～2.42ppm，雞蛋之檢出率為17.0%，殘留量0.02～0.12ppm，雞肝之檢出率為44.0% （檢出11件，其中4件超過4ppm），殘留量0.02～15.16ppm。目前我國規定肉品中不得檢出乃卡巴精。

A high performance liquid chromatographic (HPLC) method for the deter-termination of nicarbazin residues in muscle, egg and liver of chicken has been developed. Nicarbazin was extracted from samples with acetonitrile. After filtration, the extract was purified by liquid-liquid partition, concentrated by rotary evaporation, cleaned up through an alumina column, and finally determined by HPLC. Recovery studies were performed at the 0.1, 0.2, 0.4 and 0.8 ppm fortification levels in each of the three commodities. Average recoveries were 89.8-93.8% from muscle, 89.9-93.3% from egg and 90.4-92.5% from liver. Coefficient variations were less than 10%. The limit of detection was 0.02 ppm. Thirty samples of muscle, 47 samples of egg and 25 samples of liver were analyzed. In muscle N.D. (non-detectable)¬—2.42 ppm, egg N.D.—0.12 ppm, and liver N.D.—15.16 ppm of nicarbazin were found in samples. Positive detection rates among the samples of muscle, egg and liver were 33.3, 17.0 and 44.0%, respectively.