食品中不明微生物之國際能力試驗評鑑成果

The Evaluation of Performance for Detecting Pathogenic Microorganisms in FEPAS Proficiency Test in 2015

為確實做好食品衛生安全把關工作維護民眾健康，衛生褔利部食品藥物管理署（以下稱食藥署）積極參與國際知名機構舉辦之食品檢驗能力試驗。2015年參加英國中央科學實驗室主辦之食品微生物檢驗能力試驗FEPAS Proficiency Testing Program-食品中毒未知目標病原微生物之檢測，該試驗中模擬食品中毒案情境中毒症狀，受測單位需自行研判案情，並由測試檢體為「辣味牛肉飯」餐食，擬定檢驗策略進行分析，判定測試檢體中引發食品中毒之病因物質。食藥署結合傳統培養法、生化代謝鑑定、核酸分析及蛋白質質譜分析技術，於時限內成功檢出該測試檢體中含沙門氏桿菌（Salmonella spp.）、綠膿桿菌（Pseudomonas aeruginosa）及表皮葡萄球菌（Staphylococcus epidermidis）。全球共12間實驗室參加此能力試驗，其中有6間可正確判定「沙門氏桿菌」為此模擬中毒案情境之主要肇因病原，而食藥署則是唯一可準確鑑別出測試檢體中所有3種微生物的實驗室，顯示食藥署對於食品中毒病原菌及未知目標微生物之檢測能力均達國際水準。

For the purpose of food safety management, TFDA participates in accredited laboratory proficiency tests (PT) organized by well-known international institutions to evaluate the performance of personnel working in microbiological detection and analysis annually. In 2015, TFDA participated in food microbiological trials of FEPAS proficiency test program conducted by the UK Central Science Laboratory. In the trial of unknown target microorganisms, FEPAS PT program simulated the scenarios of a food poisoning case in which the symptoms of diarrhea, fever, abdominal pain were found in the latency of 12 - 72 hours. The test sample ＂spicy beef and rice meals＂ was provided for each participant to determine the trigger agent causing food-poisoning symptoms and other microorganisms also present in the test sample. TFDA developed a strategy for analyzing unknown microorganisms, in which traditional culture, biochemical identification, nucleic acid analysis and proteomic technology were combined for multi-check the species and features of microorganisms found. Within the limited time, TFDA successfully identified Salmonella spp., Pseudomonas aeruginosa and Staphylococcus epidermidis from the test sample, and convicted Salmonella spp. as the major trigger agent. A total of 12 laboratories participated in this PT program, and six of them determined ＂Salmonella spp.＂ was the causing agent of this food poisoning case. However, only TFDA accurately identified all 3 inoculated pathogens and displayed the professional ability in detecting unknown microorganisms.