比較三七皂苷、多糖體和水層抽取物對人類精子活動力的體外活性效應

Compared the Active Effects of Panax Notoginseng Saponin, Polysaccharide, and Aqueous Fraction on Human Sperm Motility in Vitro

本研究目的旨在探討皂苷、多糖體和水層等三種三七之抽取物對體外人類精子活動力的體外活性效應。精液樣本依世界衛生組織之精液分析標準，分為正常組 15 例與異常組23例，正常組的精子濃度每西西大於兩千萬隻，活動力大於50%；異常組的精子濃度每西西大於每千萬隻，活動力小於 50%。實驗分 F-10 溶液對照組與三七溶液治療組，三七溶液分皂苷、多糖體和水層抽取物等三種，每種溶液分 1.0 mg/ml 和 2.0 mg/ml 等兩種濃度，實驗時精液樣本均經由洗滌的方式處理，然後用美國漢彌爾頓電腦輔助精液分析儀（HTM-2030）觀察三七之三種抽取物溶液在 60 分鐘與 120 分鐘，對精子「活動力」與「前進活動力」的體外活性效應。實驗結果顯示，三七之三種抽取物，濃度 1.0 mg/ml 和 2.0 mg/ml 對正常組與異常組精子「活動力」的體外刺激活性，在 60 分鐘與 120 分鐘時，均有明顯刺激精子活動力的作用；就濃度效應而言，三七皂對異常組和三七多糖體對正常組精子「活動力」的促進作用；就濃度效應而言，三七皂對異常組和三七多糖體對正常組精子「活動力」的促進作用，在 120分鐘時 1.0 mg/ml 顯著優於 2.0 mg/ml。三七之三種抽取物 1.0 mg/ml 和 2.0 mg/ml 對正常組與異常組精子「前進活動力」的體外刺激活性，在 60 分鐘時，除了異常組三七皂2.0 mg/ml 之濃度外，均有明顯的促進作用；120 分鐘時，正常組僅三七多糖體濃度 1.0mg/ml 對精子「前進活動力」有增進作用；異常組除了三七皂濃度 2.0 mg/ml 外，對精子「前進活動力」也有顯著增進作用。

The purpose of the present study was to investigate the biological activity effects of Panax notoginseng saponin, polysaccharide, and aqueous fraction on human sperm motility in vitro. the study was based on 38 eligible semen samples performed by the computer assisted semen analyzer according to the semen criteria in the WHO. Fifteen samples with a concentration over 20 million per mililiter and a motility over 50% placed in the normal group, and 23 semen samples with the same concentration and a motility less than 50% placed in the anomalous group. Each sample was prepared by a washing method, before observing the effects of 1.0 mg/mL and 2.0 mg/mL Panax notoginseng fractions solutin on sperm motility as well as on progression at 60 minute and 120 minute. The results demonstrated that the stimulating activity of 1.0 mg/mL and 2.0 mg/mL of Panax notoginseng fractions solution on sperm motility produced a significant increase in the normal and anomalous group at 60 minute and 120 minute in vitro. As for the concentration effect, the 1.0 mg/mL of Panax notoginseng saponin solution, in the anomalous group, and polysaccharide solution, in the normal group that enhanced the sperm motility, was markedly superior to the 2.0 mg/mL at 120 minute. the stimulating activity of 1.0 mg/mL and 2.0 mg/mL of Panax notoginseng extracts solutin on sperm progression had an obviously significant enhancement, except the 2.0 mg/mL solution of saponin solution in the anomalous group at 60 minute in vitro. At 120 minute, only the 1.0 mg/mL of polysaccharide solution, in the normal group, that increased the sperm progression, was significantly different from the F-10 control group. In the anomalous group, Panax notoginseng extracts solution had a significant increase of sperm progression except the 2.0 mg/mL of saponin solution.