補中益氣湯對精子活動力的影響評估

Effect of Bu-Zhong Yi Qi Tang on Human Sperm Motility

實驗室精子的活動分析，比較客觀可靠的方法，主要有過膜游動法與電腦輔助精液分析法，本實驗以補中益氣湯抽取物，用上述二項方法，進行對精于游動力的影響評估。實驗先以過膜游動法觀察補中益氣湯對精蟲游動力的影響，結果發現，對「正常組」的刺激無統計意義，而「異常組」以1.0mg 、0.1mg 、0.001mgjml 濃度的刺激作用，具有統計意義，而電腦輔助精液分析法的統計結果，亦與此相同。過膜游動法所測得的精蟲前進活動百分比（前進活動力精蟲數目／精蟲總數；progressivepercent），為迅速向前游動的精子，其與男性致孕能力有密切關係。本實驗用美國TheHamilton Thorn Research 所開發的電腦輔助精液分析儀，機型為HTM-2030，本儀器的特點，對前進活動性精蟲有一診斷標準(progressive criteria)，即精子游動線性指標比值(計划。tness，STR)>80%，精子游動路徑的常態速度(Path velocity, VAP) > 25 mirco/sec，電腦軟體可以計數前進活動的精蟲數目和百分比。實驗中篩選了九例屬中醫辨證脾肺氣虛者，其精蟲前進活動力百分率< 40%為研究對象，結果發現補中益氣湯能增強前進性精蟲的百分率，其中以0.1mg 和0.01mgjml 的濃度具有統計學的意義。

The effect of Bu Zhong Yi Qi Tang (BZYQT) on the human sperm motility was evaluated using the more objective sperm motility assessment methods of transmembrane migration method (TMM) and computer-assisted semen analysis (CASA). TMM analyses indicated that while 'normal' sperms were not affected, 'abnormal' sperms were significantly stimulated by 1.0mg, 0.1mg and 0.001mg/ml of BZYQT. CASA yielded similar results. The TMM index (progressive sperms/total sperms in units) is closely correlated to male fertility. Using the sperm velocity (V) of 25 micros/second and straightness (S) of 80% as the progressive criteria, analysis with the Hamilton Thorn Research Analyser indicated 9 cases with original sperm progressive index of ＜40% were improved in vitro with 0.1mg and 0.01mg/ ml of BZYQT.