雷公藤及十全大補湯對自體免疫老鼠NZB/NZW F1之影響

Immunolocial Effect of Extracts of Tripterygium Wilfordii and Shih-Chuan-Da-Bo-Tang in NZB/NZW F1 Mice

自體免疫疾病是由免疫異常引起的，為因不明。一般而言T 和B 細胞的過度反應，及產生自體抗體，為其特性。現認為抗雙股核酸抗體IgG 類者，能與腎組織作用，故可能與人類及老鼠紅斑狼瘖引起的腎孟炎有關。在本研究中，我們探討中醫藥治療自體免疫疾病的呆效。我們以NZB/NZW F1 小老鼠的病變、尿蛋白、抗核酸抗體、免疫增生反應及血球數目，為實驗的指標。文獻上有以活血化瘀治紅斑狼瘡，以雷公藤為例。其中含有抑制免疫作用的成份。另有以滋養調補來治療的，以十全大補湯為例。這兩種藥，以水煎法製備。濃縮乾煒後冷凍於-70.C 。小老鼠滿月後，每週給藥三次或五次，達六個月以上。觀察記錄其病變。實驗結束時，殺死老鼠，取其血液脾臟樣品分析。結果我們發現，雷公藤及十全大補湯治療後，小老鼠的死亡率比對照組略為降低。而尿蛋白及抗核自主抗體，在實驗二中有顯著改善(P<0.05)。雖然十全大補湯具補血作用，可維持血球數目，但對老鼠免疫增生反應，並無明攝影響。雷公藤僅有些抑制免疫作用。因此需要更進一步的實驗，來肯定這兩種藥對自體免疫老鼠的功效。

The effect of extracts of Tripterygium wilfordii (TW) and Shih-Chuan-Da-Bo-Tang (DT) in autoimmune-prone NZB/NZW H mice was studied. The results showed that these treatments have some beneficial effects to the survival and immune parameters in the mice. The mortality rate was reduced slightly (P＞0.05) in the mice receiving treatment with DT or WT as compared with that of the control. Nephritogenic IgG levels of anti-DNA antibody and proteinuria from the TW treated mice were reduced significantly (P＜0.05) in one experiment. Lymphoproliferative responses to mitogens were modulated by the treatments with TW and DT, although not significantly. Further studies are warranted before a conclusion can he reached as to the effects of TW and DT.