台灣禮樂煉銅廠附近五節芒之超氧歧化酶的研究

Studies on the Activity of Superoxide Dismutase in Miscanthus floridulus Grown near a Copper Smelter in Taiwan

以植被梯度分析方法，探討台灣北部禮樂煉銅廠產生之污染對當地植被之影響。由其殘存物種之種歧異度和出現頻度，顯示附近之植被有明顯之梯度變化。五節芒為煉銅廠主要優勢種之一，以其作為偵測當地植被對逆境梯度反應之指標，並研究其超氧歧化酶（SOD）之同功酶形式與當地逆境梯度之關係。五節芒葉片中含有MnSOD及CuZnSOD多種，其MnSOD活性隨著當地逆境加強而遞增，最後討論五節芒之MnSOD作為煉銅廠污染之生化指標之可行性。

A simple gradient transect of vegetation analysis was used to study the effect of the pollution caused by a copper smelter in northern Taiwan on the development of vegetation in the area. A clear coverage gradient on the vegetation was found near Liiyueh copper melter after ten years of operation, and an apparent stress gradient was evident in a series of plots in the vicinity on the basis of species diversity and frequency of vegetation found. Miscanthus floridulus was a good indicator plant to gauge the vegetation's response to the stress gradient, and its isozyme pattern of superoxide dismutase was studied in connection with this stress gradient. Four MnSODs and five CuZnSODs were present in the leaves of Miscanthus floridulus. Its SOD activity, and particularly its MnSODs, showed obvious accumulation in the stress area. MnSOD of Miscanthus floridulus as a biochemical indicator for copper smelter pollution is discussed.