利用隨機複製DNA標誌分析台灣產煙麴黴之不同溫度品系

Use of Random Amplified Polymorphic DNAs Markers to Distinguish Temperature Isolates of Aspergillus fumigatus of Taiwan

煙麴黴種內之十株溫度品系，四株分離自台灣，及六株來自各菌種中心（CBS，ATCC及IMI）所收集對溫度反應各異的菌株，以隨機複製DNA標誌（RAPDs）做種內變異之分析。在10組的引子中，有5組引子顯示明顯的差異。實驗的結果將所有的煙麴黴品系區分成四群，且與其生理特性相吻合。以RAPDs的分析方式，可以區分出煙麴黴種內溫度變異之品系，並可以將其區分成不同的溫度群。

Four temperature isolates of Aspergillus fumigatus Fres. from Taiwan and six isolates from several culture collection centers (CBS, ATCC and IMI) were analyzed by random amplified polymorphic DNAs (RAPDs). Ten oligonucleotide decamers were used to generate selective markers from genomic DNAs and live primers gave adequate discrimination between isolates. All isolates were classified into four groups by RAPDs that consistent to their physiological characteristics. The results indicate that RAPDs analysis is useful to assess the genetic diversity and intraspecies temperature variation among different temperature strains of Aspergillus fumigatus.