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篇名 |
利用隨機複製DNA標誌分析台灣產煙麴黴之不同溫度品系
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並列篇名 |
Use of Random Amplified Polymorphic DNAs Markers to Distinguish Temperature Isolates of Aspergillus fumigatus of Taiwan |
作者 |
陳懋彥 (Mao-Yen Chen)、陳桂玉 (Guey-Yuh Chen)、蔡珊珊 (San-San Tsay)、陳瑞青 (Zuei-Ching Chen) |
中文摘要 |
煙麴黴種內之十株溫度品系,四株分離自台灣,及六株來自各菌種中心(CBS,ATCC及IMI)所收集對溫度反應各異的菌株,以隨機複製DNA標誌(RAPDs)做種內變異之分析。在10組的引子中,有5組引子顯示明顯的差異。實驗的結果將所有的煙麴黴品系區分成四群,且與其生理特性相吻合。以RAPDs的分析方式,可以區分出煙麴黴種內溫度變異之品系,並可以將其區分成不同的溫度群。 |
英文摘要 |
Four temperature isolates of Aspergillus fumigatus Fres. from Taiwan and six isolates from several culture collection centers (CBS, ATCC and IMI) were analyzed by random amplified polymorphic DNAs (RAPDs). Ten oligonucleotide decamers were used to generate selective markers from genomic DNAs and live primers gave adequate discrimination between isolates. All isolates were classified into four groups by RAPDs that consistent to their physiological characteristics. The results indicate that RAPDs analysis is useful to assess the genetic diversity and intraspecies temperature variation among different temperature strains of Aspergillus fumigatus. |
起訖頁 |
201-206 |
關鍵詞 |
煙麴黴、隨機複製DNA、種內溫度變異、Aspergillus fumigatus、RAPDs、Intraspecies temperature variation |
刊名 |
TAIWANIA |
期數 |
199709 (42:3期) |
出版單位 |
臺灣大學生命科學院;台灣生物多樣性協會
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該期刊-上一篇 |
台灣禮樂煉銅廠附近五節芒之超氧歧化酶的研究 |
該期刊-下一篇 |
臺灣南部南仁山森林之小苗形態 |
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