利用兩段式電泳分析分離絲瓜韌皮部42.5kDa蛋白質

Separation of a Phloem Specific 42.5 kDa Protein with Two-Dimensional Gel

絲瓜果實經刀片輕切裂口，塗上含抗氧化劑的萃取液後，其與韌皮部溢流物一起收集。以混合液經活性與變性（SDS）的兩次電泳分析。一維分離獲得85 kDa活性蛋白質，其約佔全蛋白質的28.2%，其在二維電泳膠上呈五條帶，其中的42.5 kDa的變性蛋白質在高效能電泳色層分析顯示單一峰帶，而在變性銀染色膠上僅呈一蛋白質帶。

Phloem exudates leaking from Luffa cylindrica fruits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimensional gel. i. e., native and sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE). A major protein of 85 kDa occupying 28.2% of total protein had 5 bands on denatured gel. One of them with molecular weight at 42.5 kDa was complementally identified by reversed-phase high performance liquid chromatography and silver stained-band on SDS-gel.