香茹正己烷萃取液抑制T細胞活化之研究

n-Hexane Extract from Glossogyne Tenuifolia Inhibits T Cell Activation

香茹(Glossogyne tenuifolia, GT)是傳統的中草藥具有抗發炎的活性。為了了解香茹對於免疫調節的能力，我們測試香茹對T細胞誘發的適應性免疫力的影響。在本研究，我們發現香茹正己烷萃取液不會造成人類周邊血單核細胞(human peripheral blood mononuclear cells; PBMCs)死亡，而且展現抑制人類周邊血單核細胞增殖及產生γ干擾素(interferonγ；IFNγ)的能力。進一步分析，我們發現香茹正己烷萃取液會抑制植物血凝素(Phytohaemagglutinin, PHA)刺激人類周邊血單核細胞活化裂質素蛋白激酶(Mitogen-activated protein kinases, MAPKs)，ERK2、p38及JNK的磷酸化。我們的研究顯示香茹正己烷萃取液藉著抑制MAPKs的活性，調節T細胞之活化。

Glossogyne tenuifolia (GT) is a traditional Chinese herbal medicine previously shown to exhibit anti-inflammatory activity. To explore the immune-regulating power of GT, we tested the effect of GT on T lymphocyte-mediated adaptive immunity. In this study, we obtained the n-hexane fraction of GT possessing suppressive potential in PHA-stimulating human peripheral blood mononuclear cells (PBMCs) proliferation and secretion of interferonγ(IFNγ). We demonstrate herein that the n-hexane fraction of GT inhibited T cell activation, which was not due to its cytotoxic activity. In addition, the n-hexane fraction of GT significantly reduced mitogen-activated protein kinases, extracellular signal-regulated kinases (ERK), p38 and c-Jun N-terminal kinases (JNK), phosphorylation in a T cell activation-dependent manner. Therefore, decreased MAPKs activation mediated the downrreguated effect of GT in T cell activation.