This study explores the effects of light source on the imagery of guava to understand how light source design can be used to make fruits feel pleasing. First, using questionnaire analysis and cluster analysis, six fruit image vocabulary were obtained. Based on combinations of color temperature (2700°K, 4000°K), color rendering (70 CRI, 80 CRI), and illuminance (750 Lux, 1500 Lux), 8 LED light sources were designed. Then, under these light sources, experiments of guava preference for six images and overall satisfaction were conducted. The obtained evaluation data was further analyzed by the analysis of variance and the correlation analysis. The results show that combinations of the light source have significant effects on the evaluation of the overall satisfaction. The participants reported the worst overall satisfaction when the light source is at color temperature of 2700 (°K), color rendering (80CRI), and illuminance of 750 (Lux). Thus, a combination of color temperature (4000°k), color rendering 70 (CRI), and illumination 1500 (Lux) was proposed to improve the overall satisfaction of the testers. In addition, all the six images are significantly correlated with the overall satisfaction, the correlation of the four images of fresh, fine taste, crystal clear and crisp is high, but those of sweet and sour and rich aroma is low. The results show that the use of light source design can create a special image of fruit, giving people positive psychological awareness and feelings. The results of this study can be used as a reference for light design as a basis for improving lighting conditions.