The arteriovenous (A-V) shunt is the main pathway for hemodialysis in patients with end stage renal failure. In a medical center from January to March in 2013, two patients had A-V shunt bleeding after hemodialysis and it affected the function of fistula. After data collection, we found the possible reasons of the accident were as follows: (a) The patients and nurses lacked the knowledge on how to prevent A-V shunt bleeding; (b) There was no standard protocol to control the hemostasis of A-V shunt after hemodialysis; (c) There was no teaching instrument to measure the hemostatic pressure of A-V shunt. To effectively improve the condition of bleeding in A-V shunt, we provided solutions for patients and nurses. (a) To offer clinical guidance for hemostasis; (b) To make reminder cards of hemostasis; (c) To provide arteriovenous fistula care on-the-job education; (d) To educate clinical nurses on arteriovenous fistula hemostasis; (e) To amend the rules of arteriovenous fistula care; (f) To formulate new education guideline for patients; (g) To develop the teaching instruments. The project was implemented in June 2013. In November of the same year, 400 dialysis patients were surveyed to evaluate the implementation results of this program. The results showed that the rate of A-V shunt bleeding after hemodialysis decreased from 19.9% to 4.5%, and continued to track until 2014, the results from all the ensuing months were below the standard value of 6%. Finally, it is hoped that the content provided by this project will help hemodialysis patients to have better medical quality in clinical treatment.