The central venous catheter (CVC) procedure is a crucial and necessary medical treatment for patients with critical illness, but it is also a key risk factor for bloodstream infection. The CVC bundle care can decrease the risk for central line-associated bloodstream infection (CLABSI). However, the CLABSI rate in our medical unit was 6.78‰, with an increasing trend from 2012 to 2013. The average CVC indwelling time was up to 20 days and the accuracy rate of optimal CVC care was only 65%. Our analysis showed that these suboptimal practices were related to our nursing staff’s unfamiliarity with central line placement procedures and lack of related skills and knowledge regarding central line care. The goals of the project were to decease CLABSI to 4.51‰ and increase the accuracy rate of CVC bundle care to 90% in order to ensure the patients’ safety. To achieve these goals, we developed the CVC bundle care mobile application and implemented the corresponding on-the-job training to apply to our inpatient care. We also evaluated the nursing staff’s CVC bundle care skills to assess the project outcomes. After implementing the project, the rate of CLABSI decreased from 6.78‰ to 3.94‰, the average CVC indwelling time decreased from 20 days to 14 days, and the accuracy rate of CVC bundle care increased from 65% to 95%. As these results support the efficacy of the project, implementing to all acute care units in the hospital can be considered.