Purpose: An important factor in postoperative rehabilitation and return to play decision making is to ensure appropriate restoration of lower extremity muscular strength and dynamic postural control. We can know the anterior cruciate ligament reconstruction of lower limb neuromuscular control for the individual plays an important role during landing period. Therefore, the athletes after ACLR need to have standardized rehabilitation and physical therapy program, as to functional assessment, as looking over whether the knee joint possesses the normal function. The common functional test its include eyes open, eyes closed, shuttle run, crossover step, one-legged hop and jump landing. The jump landing is commonly used to measure dynamic stability of the lower extremity. The aim of this study was to investigate the characteristics of lower extremity kinematics and muscle activations in athletes after anterior cruciate ligament reconstruction during jumping-landing maneuver. Methods: Nine participants with a history of unilateral anterior cruciate ligament reconstruction (22.5 ± 0.8 year, 170.3 ± 8.5 cm, 65.7 ± 11.3 kg; Time since surgery: 23.3 ± 16.8 months) and nine sex, age, weight, height and sport matched participants who served as the control group were recruited for this study. Participants were asked to perform a doubleleg jump followed with a singleleg landing. Ten Vicon infrared video cameras (200 Hz), and eight wireless EMG sensors (1000 Hz) were synchronized to acquire the lower extremity 3D kinematic data and muscle activation profiles during jump landing. Nonparametric test (Mann-Whitney U test) was used to compare ACLR and control group (α = .05). Results: During pre-landing phase, the ACLR group showed significantly greater gluteus medius and rectus femoris muscle pre-activation, and greater knee flexion angle compared to the control. The ACLR group demonstrated a significant less movement time then the control group during the descending phase of landing. During propulsion phase, greater gastrocnemius and tibialis anterior muscle activations were found compared to the control group. Conclusions: When performing jump-landing protocol, the ACLR group utilized more gluteus medius and rectus femoris muscle to maintain hip and knee stability during landing period. In addition, greater knee flexion angles and less movement time were adopted by the ACLR group to enhance joint stability during the decending phase. Gastrocnemius and tibialis anterior muscles were activated as the landing strategy to help stabilize the ankle.