以大腸桿菌利用甘油進行生物合成途徑產生高產率之莽草酸

High-yield Shikimic Acid Production from Glycerol by Biosynthetic Pathway in Escherichia Coli

莽草酸（3,4,5-三羥基環己烯羧酸；SA）是合成磷酸奧司他韋（Tamiflu）的關鍵中間體，它在皮膚化妝品製劑，抗酵素活性方面具有多種創新和多功能作用，並可作為去角質劑。在此研究中，使用大腸桿菌進行生產SA。藉由確認各種營養和物理參數的影響來優化莽草酸生產的過程。可從甘油獲得最大SA產量濃度（33.44g／L）和產率（43.1％）。這些結果說明，除了常用的葡萄糖外，可使用甘油作為替代碳源，以大腸桿菌生產SA。此外，這些數據將為今後研究以甘油利用該生產菌株來生產更高量的莽草酸奠定基礎。

Shikimic acid (3, 4, 5-trihydroxy cyclohexene carboxylic acid; SA) is a key intermediate for the synthesis of the oseltamivir phosphate (Tamiflu), and it has several innovative and multifunctional roles in dermocosmetic preparations, anti-enzymatic activity, and as an exfoliating agent. In this study, production SA by using E. coli was carried out. The process of producing SA was optimized by determining effect of various nutritional and physical parameters. The maximum SA production titer (33.44 g/L) and yield (43.1%) were achieved, which was the highest value ever reported from glycerol. These results suggest that utilization of not only currently used glucose but glycerol as an alternative carbon source is much effective for the production of SA in E. coli. Moreover, these data would lay the foundation for future research on the utilization of this strain from glycerol and to achieve higher SA production efficiency.