維生素B1對洋菇菌絲發育影響之研究

A Study on the Effect of Thiamine on the Growth of Mushroom Mycelium

本試驗利用在玉米培養基上純粹培養之菌絲，接種在含有0，1，2，3，4與5 ppmT hiamine 的合成培養基上，在25°C溫度下培養兩週，觀察菌絲發育之情形，測定生長六次，每次測定菌絲生長範圍，最後觀察菌絲粗度及顏色，並稱取培養後菌絲乾物重量。由測定菌絲生長蔓延面積、蔓延生長速度、菌絲粗度、顏色及菌絲脆物瓜量等結果來看，Thiamine對洋菇菌絲的生長沒有促進的效果，但由最後測定的面積與菌絲乾物產量來計算單位面積的菌絲乾物產量來看，Thiamine有增加單位面積乾物產量的痕跡，Thiamine 濃度在1-5 ppm時，濃度愈高，增加單位面積菌絲乾物產量也愈多。

The effect of thiamine on the growth of mushroom mycelium has been studied. A pure culture of mushroom mycelium was transfered from the tissue on corn-agar medium to the synthetic medium which comtained 0, 1, 2, 3, 4 and 5ppm. of thiamine and the cultures were incubated under 25°C. for two weeks. Observations were made on the growing area of mycelium during the period of incubation, and the diameter, colour and dry weight of the mycelium at the end of incubation. Mushroom mycelium was found to grow satisfactory in the synthetic medium and to be unaffected by addition of thiamine, so far as the results of exteral measurements are concerned, but the dry weight of unit area (mg./mm2.) of thiamine treated samples at the end of incubating were found much greater than the untreated samples.