金針菜之組織培養繁殖

The Propagation of Edible Daylily (Hemerocallis fulva L.) by Tissue Culture

金針菜（Hemerocallis fulva L.）短縮莖的次頂分生組織部位橫切汁，置於MS添加2.4-D及BA的半固態培養基35天後，形成的癒傷組織可直接分化不定芽或形成叢生芽，平均一片產生5.3個芽體。短縮莖去除生長點後切下，分別培養於添加BA或NAA或2.4-D的MS培養基上，結果含有BA者都能有效的促成潛伏側芽萌發，其中以含BA 1.0mg/l所產生的5.9芽為最多。花梗、花絲、花蕾基部等花部組織以MS加NAA（10mg/l）及kinetin（0.1 mg/l）的培養基培養，均能誘導產生癒傷組織，並且經繼代培養後有擬胚體之分化。

Compressed shoot slices with sub-meristem tissues of edible daylily (Hemerocallis fulva L.) were cultured on agar-solidified Murashige and Skoog's medium supplemented with 2,4-D (1.0mg/l) and BA (3.0 mg/l), calli were induced and then regenerated plantlets within 5 weeks. Latent axillary buds of the de-capitated compressed shoot block were induced in MS media supplimented with BA (0.1-5.0 mg/l), and treated with 1.0mg/l BA given highest 5.9 buds per explant. Flower scapes, filaments and pedicles can be also induced to produce callus and regenerate numerous plantlets. With these techniques, mass propagation of edible daylily through in vitro culture is feasible.