蝴蝶蘭與朵麗蝶蘭花梗節間之組織培養

Clonal Propagation of Phalaenopsis and Doritaenopsis by Internode of Flower Stalk in vitro

以蝴蝶蘭（Phalaenopsis）和朵麗蝶蘭（Doritaenopsis）幼嫩花梗節間斜切成薄片，置於1.2倍Vacin和Went之基本鹽類培養基，內含BA 1mg/1者對擬原球體之形成最有利，可獲得73.4%之成功率，BA 5mg/l者擬原球體之形成降為46.8%。含BA與NAA之組合者均降低擬原球體之形成，添加kinetin，kinetin+NAA或2，4-D之各種生長調節劑之組合中，經培養之莖片均黑化死亡。以含BA 1mg/l之基本培養基，培養蝴蝶蘭及朵麗蝶蘭不同在便發育階段各節間切片擬原球體之形成，結果顯示由花梗可見至45天以前之全部幼嫩花序及各發育期花梗頂端表皮細胞等具分裂能力之節間組織，最有利於誘發擬原球體之形成，共成功率可達62.9-77.1%不等。第一花蕾可見以後各發育期，隨著花梗發育愈久，分化擬原球體比率也愈下降，能做為培養體之節間也愈短。當花謝後整枝花梗節間即不再具有分化擬原球體之能力。

Clonal propagation of Phalaenopsis and Doritaenopsis by internode of flower stalka in vitro were studied. 73.4% protocorm-like bodies (PLB) formed from epidermal cells, and/or parenchyma cells near the vascular bundles of internode tissue was found in the 1.2 times Vacin and Went's basal salts, with 1 mg/I BA. However, only 46.8% PLB formed when BA content increased to 5 mg/I. BA+NAA, decreased PLB formation. Kinetin+NAA or 2, 4-B, all thin slices of internodes blacked and necrotic. Subculture the PLB could get large number of PLB, and formed young plants thereafter. Young flower stalk with dividing epidermal cell and parenchyma cell near the vascular bundles are easy to form protocorm-like bodies in some species and hybrides of Phalaenopsis and Doritoenopsis. After the last floret anthesis, the mature internodes were not easy to form PLB in BA 1 mg/I medium. It seems likely, the longer time of flower stalks extend, the less possibility for cultured internodes will be, and induced the PLB formation percentage will decrease. After the last flower senescences, all internodes do not have ability of differentiation.