臺灣一葉蘭之組織培養

Tissue Culture of Pleione formosana Hayata

臺灣一葉蘭行組織培養，以秋末落葉採收之成熟碩大球莖，經1至2個月5°C冷藏後，取其花芽內莖頂為培植體最易成功。1/3 MS鹽類促進原球體的形成與增殖，在Knudson'sC培養基下易有不定根之發生、原球體生成數少。NAA 1 ppm或低濃度2,4-D有助於原球體之增生，當球莖冷藏後取花芽內之莖頂繁殖時，植物生長調節劑並非絕對必霈。椰子水並無顯著效果，固體培養方式似優於液體培養。臺灣一葉蘭經組織培養可正常增殖、結球，其繁殖速率比無菌播種之實生繁殖為快。

The most suitable tissue of Pleione formosana Hayata for tissue culture was the shoot tip from flower bud of large and matured corm, which was harvested in winter and then stored at 5°C for one to two months. Somatic protocorm formation and proliferation from shoot tip were promoted by 1/3 MS basal salts. However, adventitious roots induced easily and few somatic protocorms were formed from shoot tip cultured on Knudson's C medium. After cold storage, the shoot tips had little response to plant growth regulators for somatic protocorm proliferation. The coconut milk did not make a significantly positive effect. The proliferation of shoot tip seemed to perfrom better in solid media than in liguid ones. The growth of plantlets and bulbing through tissue culture were faster than seedlings germinated from seeds.