觀賞石斛利用擬原球體大量繁殖

Mass­propagation of Ornamental Dendrobium from Protocorm Like Body (PLB)

本研究探討觀賞石斛（DendrobiumEkapal｀KingPanda’）擬原球體（protocormlike body，PLB）大量繁殖、芽體形成及發根之影響因子。橫切之PLB培植體於添加2mg／LNAA，IBA，kinetin，BA或TDZ生長調節劑之基本培養基（MS基本鹽類添加80g／L馬鈴薯、30g／L蔗糖及9g／L洋菜）中培養，結果顯示細胞分裂素（cytokinin）較生長素（auxin）對PLB增殖有提高之作用，其中以2mg／Lzeatin及BA處理之PLB增殖率最高，約70%之培植體皆可增殖16個以上之PLB。進一步將上述試驗所得之PLB單顆接種於含有80g／L豆薯或馬鈴薯的基本培養基中，比較有機添加物對PLB之芽體形成之影響，結果顯示自2mg／Lzeatin處理誘導所得之PLB，接種在豆薯培養基中有最高之芽體形成率；而自IBA處理中所誘得之PLB在豆薯及馬鈴薯培養基中皆有高比例之芽體形成率。發根試驗中探討培養基中MS基本鹽類濃度及繼代培養時進行切根處理對小苗發根生長之影響，結果顯示含有1／4 MS基本鹽類濃度之培養基發根率最高，而繼代時之切根處理，會影響組培苗地上部之苗長及不定芽數，但對地下部之根長及根數影響並不顯著。 This study is to investigate mass propagation of the protocorm like bodies (PLB) of ornamental Dendrobium (Dendrobium Ekapal ‘King Panda’). The PLBs of ornamental Dendrobium were transversely cut and adopted as explants. They were cultured on the MS basal salt media containing 30 g/L of sucrose, 80 g/L of potato homogenate, 9 g/L of agar and in combination with 2 mg/L of different kinds of plant growth regulator such as NAA, IBA, BA, kinetin, TDZ and zeatin for PLB proliferation investigation. After 30 days, cytokinin was superior to auxin for PLB proliferation. The highest PLB multiplication rate was obtained on the media containing 2 mg/L of zeatin or BA, about 70% of the explants generating 16 or more PLBs in a month. The PLBs proliferated from the above experiment were subcultured one by one on the MS basal salt media with the organic compound made of yam bean or potato homogenate for shoot formation investigation. After 60 days, PLBs, from the zeatin treatment, subculture on the medium containing yam bean had the highest shoot formation rate. The shoot formation rate of IBA­induced­PLBs was high, no matter they were cultured on yam bean or potato homogenate containing media. The 1/4 diluted MS basal salt medium had the best root induction and growth rate. Root cutting affected plantlet height and the number of shoot. However it had no significant influence on root length and number of root.