Aesculus hippocastanum 對乳癌細胞 MCF-7 的抗氧化能力與細胞毒性   全文下載

Antioxidant Capacity and Cytotoxicity of Aesculus hippocastanum on Breast Cancer MCF-7 Cells

Aesculus hippocastanum L. 係一亞洲原生樹種。它的花、葉及種子長久以來被用作民俗醫療與傳統食材。本研究以乙醇萃取A. hippcastanum的樹皮、種子、葉及花等地上部分。測定每一部位的抗氧化能力，包括對1,1-diphenyl1-2-picrylhydrazyl（DPPH）自由基的清除能力、微粒體脂質過氧化抑制能力及總酚類含量。在所有的部位中，樹皮的萃取物展現了最高的抗氧化能力，其IC50值對脂質過氧化為0.025 mg/mL及DPPH自由基消除為0.014 mg/mL。利用MTT技術，樹皮的萃取物進一步測定其對於人類乳癌細胞株（MCF-7）和健康細胞（3T3）的細胞毒性，添加 0.5 mg/mL樹皮萃取物會使兩種細胞的存活度皆降至30%。"

Aesculus hippocastanum L. is a native tree of Asia. Its leaves, seeds and flowers have long been used in folk medicine and in traditi-onal food ingredients. In this study, the bark, seeds, leaves and flowers as aerial parts of A. hippocastanum were extracted in ethanol. The antioxidant capacity of each part was determined for its 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging capacity, microsomal lipid peroxidation inhibition capacity and total phenolic content. Among all the parts examined, the bark extract of A. hippocastanum revealed the highest antioxidant capacity with an IC50 value of 0.025 mg/mL and 0.014 mg/mL for the inhibition of lipid peroxidation and for the scavenging of DPPH radical, respectively. The bark extract was further examined for its cytotoxic effect on human breast cancer cells (MCF-7) and on healthy cells (3T3) using the MTT method. Cell viability was reduced to 30% upon the addition of 0.5 mg/mL bark extract for both cell lines.