發芽米萃取物對人類白血病細胞 U937 生長抑制之影響及其免疫活性   全文下載

Inhibitory Effect on Human Leukemic U937 Cell Growth and Immunomodulatory Activity of Germinated Brown Rice

本研究以不同溶劑（磷酸緩衝溶液、鹼溶液及乙醇溶液）進行發芽米之萃取，以探討萃取物對人類單核細胞之增生、細胞激素（IL-1β 、IFN-γ及TNF-α）和一氧化氮的分泌及對人類白血病細胞U937生長抑制之影響。結果顯示，發芽米三種溶劑萃取物能顯著的抑制U937細胞的生長，其抑制率皆為50%以上，而且在濃度為1000 μg/mL時，三種萃取物對U937細胞生長之抑制率皆超過60%。在刺激細胞激素分泌方面，以磷酸緩衝溶液萃取所得之萃取物與單核球細胞培養3天後所得之單核細胞條件培養液，在濃度為1000 μg/mL時，其IL-1β 、TNF-α及IFN-γ之分泌量分別為1798、2347和1462 pg/mL。利用乙醇溶液所得之萃取物亦能顯著刺激單核球細胞之增生。本研究之結果可作為發芽米健康食品開發之參考。

This study was conducted to investigate how germinated brown rice (GBR) extracts stimulate human mononuclear cell (MNC) growth, secretion of cytokines (IL-1β, IFN-γ and TNF-α) and nitric oxide, and subsequently inhibit the growth of U937 cells. The results indicate that through the use of phosphate buffer solution (PGBR), alkali (AGBR) and ethanol (EGBR), the extracts had a significant inhibitory effect on U937 cell growth. Ultimately, the extracts produced an inhibition rate of over 50%. As the concentration was increased up to 1,000 μg/mL, the U937 cell growth inhibition for the three extracts surpassed 60%. The cytokines (IL-1β, TNF-α and IFN-γ) expression of PGBR-MNC-CMs (conditioned mediums) for 3 days (at 1000 μg/mL) was 1798, 2347 and 1462 pg/mL, respectively. The EGBR showed significant stimulation on the growth of MNC. The results of this study can be used as references for the development of GBR health foods.