| 中文摘要 |
納豆為日本傳統豆類發酵食品，納豆激?（Nattokinase）是納豆各種成份中最具備有效功能的成份，本研究之目的在探討納豆激?對急性缺血性中風之神經保護作用，本實驗以沙鼠為對象，並分為控制組、缺血性中風組及缺血性中風並灌食不同劑量之納豆激?組（4及40 mg/day，連續灌食7日），實驗當天除了控制組外，將沙鼠單側頸總動脈及中腦動脈結紮，缺血1小時後再灌流，造成急性缺血性中風動物模式，動物犧牲後，利用2, 3, 5-triphenyltetrazolium chloride（TTC）染色，以評估腦梗塞的體積。另一組實驗測量纖維素原（Fibrinogen）、凝血?原時間（Prothrombin Time, PT）及部分活化凝血?原時間（Activated partial thromboplastin time, APTT），以評估納豆激?對凝血系統的影響。本實驗結果發現，給予不同劑量之納豆激?的兩組其梗塞體積較缺血性中風組減少54-68%，在凝血系統測試中之凝血?原時間在各組中均無變化，部分活化凝血?原時間及纖維素原在缺血性中風組均有顯著增加，不同劑量之納豆激?的給予均能反轉缺血性中風對部分活化凝血?原時間及纖維素原之增加，目前的實驗數據顯示灌食7日純納豆激?會改變凝血參數及能提供沙鼠急性缺血性中風之神經保護作用。" |
| 英文摘要 |
Natto is a traditional Japanese food prepared from fermented soybeans. nattokinase (NK) is considered one of the most active func-tional ingredients in natto. The present study evaluated the neuroprotective effects of NK in gerbils subjected to focal cerebral ischemia. The gerbils were separated into four groups: control group, ischemic group and two NK groups (4 and 40 mg/day i.g. for 7 days). All gerbils were fed standard chow with or without NK prior to cerebral ischemia. Focal cerebral ischemia was induced by the occlusion of the right common carotid artery and middle cerebral artery for 60 min, followed by reperfusion. Infarct volumes were determined by 2,3,5-triphen-yltetrazolium chloride staining. Anti-thrombotic effects of NK, such as fibrinogen, prothrombin time and activated partial thromboplastin time, were also determined. Our data showed that NK significantly reduced infarct volumes by 54-68% (p < 0.01), compared to the isch-emic group. Prothrombin time was not significantly different in all groups. However, low dosage NK (4 mg/day) reversed ischemic-induced increase in fibrinogen concentrations and activated partial thromboplastin time. In summary, the data indicated that oral intake of NK for 7 days may alter fibrinolytic activity and provide neuroprotective effects against focal cerebral ischemia in gerbils. |
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