不同部位葛根之抗氧化活性及其類異黃酮的含量   全文下載

Antioxidant Activity and Isoflavonoid Components in Different Sections of Pueraria lobata Root

本研究主要探討不同部位葛根，包括完整葛根、葛根外皮及去皮葛根中的抗氧化活性及類異黃酮含量。樣品中類異黃酮含量利用高效能液相層分析法進行測定，抗氧化活性評估包括總酚含量、DPPH自由基補捉能力, ABTS自由基捕捉能力及還原力。由實驗結果發現，葛根外皮較完整葛根及去皮葛根明顯含有高的類異黃酮含量（p < 0.05），葛根外皮較完整葛根及去皮葛根明顯含有高的抗氧化活性類異黃酮含量（p < 0.05）。不同部位的葛根的類異黃酮都含Puerarin, daidzin, daidzein, genistin及genistein，其中的高含量的puerarin與抗氧化活性呈相當高的相關性。"

The isoflavonoids content and antioxidant activity in different sections of Pueraria lobata roots, including the root outer bark, kudzu root and whole root were investigated in this study. Isoflavonoid concentrations in P. lobata roots were determined by high performance liquid chromatography. The antioxidant activity of P. lobata roots was assessed by total phenol content, DPPH free radical scavenging assay, ABTS free radical scavenging assay and reducing power. The root outer bark of P. lobata root possessed higher isoflavonoids content than whole root or kudzu root (p < 0.05). The levels of antioxidant potential of the root outer bark assayed by total phenolic content, DPPH, ABTS and reducing power were significantly higher than those of the whole root or the kudzu root (p < 0.05). The main isoflavonoids in all sections of P. lobata roots were puerarin, daidzin, daidzein, genistin and genistein. The major isoflavonoid puerarin in root outer bark exhibited the greatest antioxidant activity in P. lobata roots.